Elisa Reader And Washer Principle

Elisa is a plate based assay technique.

Elisa reader and washer principle.

Elisa stands for enzyme linked immunosorbent assay. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. What an elisa reader does. The elisa reader reads the plate.

Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule. The illustration below shows a workflow for a typical sandwich elisa assay. The enzyme activity is measured using a substrate that changes color when modified by the enzyme. Readers need to have appropriate filter 650 nm and 450 nm.

The test is done in an elisa plate also known as a 96 well plate or microplate. Are available as fixed as well as adjustable volume as well as single channel and multi channel. An immune reaction i e. The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically.

This ability to wash away non specifically bound materials makes the elisa a powerful tool for measuring specific analytes within a crude preparation. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present.